General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CCR10 Activated GPCR Internalization Assay, 2pl Kit. (93-0783E3CP5M)
The PathHunter CCR10 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR10 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR10. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR10-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress FGR Activity Assay, 10-Plate Kit. (93-0884E3CP16L)
The PathHunter eXpress FGR Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the FGR kinase domain. The cell line is engineered to express the catalytic domain from FGR fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the FGR catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress XBP1 Nuclear Translocation Assay, 2-Plate Kit. (93-0857E3CP5M)
The PathHunter eXpress XBP1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the IRE1/XBP1 pathway. This kit assesses ligand (e.g. thapsigargin) based pathway activation via detection of resulting XBP1 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged XBP1 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of XBP1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADCYAP1R1 Total Internalization Assay, 2pl Kit. (93-0885E3CP0M)
The PathHunter ADCYAP1R1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of ADCYAP1R1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged ADCYAP1R1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADCYAP1R1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GHR-JAK2 Functional Assay, 2-Plate Kit. (93-0829E3CP14M)
The PathHunter GHR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses GHR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged GHR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Mouse RBP4 ASY
The R-PLEX Mouse RBP4 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures RBP4 levels in mouse serum plasma and tissue culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and diluents needed to perform the assay The kit can quantitatively measure RBP4 levels with high precision and accuracy It is suitable for studying RBP4 biology and evaluating RBP4 as a biomarker
Meso Scale Discovery U-PLEX Mouse IL-9 ASY
The Meso Scale Discovery U-PLEX Mouse IL-9 Assay Kit is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 9 (IL-9) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of mouse IL-9 with a lower limit of detection of 0 3 pg/mL and an upper limit of 10 000 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to MSD SULFO-TAG calibrators and sample diluent The assay takes approximately 3 5 hours to complete after a 2 hour incubation
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 96 Assay Points
HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 96 Assay Points
Cayman Chemical D-Lactate asy KIt 96Wel
D(−)-Lactate is a stereoisomer of lactate and is present in blood at only 1-5% the concentration of L(+)-lactate. Exogenous sources of D-lactate include fermented foods such as yogurt, sauerkraut, and pickles. D-lactate is normally produced in the gastrointestinal tract, mainly by lactobacilli and bifidobacteria or is produced in the cytosol of cells by the glyoxylase pathway. Cayman’s D-Lactate Assay provides a fluorescence-based method for detecting D-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, D-lactate dehydrogenase catalyzes the oxidation of D-lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Cayman Chemical Cel-Based asy nutrophIl I 25mL
An ionizable cationic lipid (pKa 6.5) has been used in the generation of LNPs for the delivery of mRNA and anticancer agents in vivo LNPs containing DMT7 and encapsulating mRNA encoding Il-12 and MSA-2 decrease tumor volume lung metastatic nodule numbers and lung levels of CD4 and CD8 T cells in a 4T1 breast cancer mouse xenograft model of lung metastasis the same LNPs decrease the number of Tregs increase the number of tumor-associated M1 macrophages and increase intratumoral levels of Il-12 Il-2 Tnf-a and Ifn-? in a B16/F10 murine melanoma model
Cayman Chemical LIPID Drop asSAy Wash Sol 30mL
A BET BD2 inhibitor (IC50s 31.62 15.85 15.85 and 39.81 nM for BRD2 BRD3 BRD4 and BRDTBD2 respectively) selective for BD2 in BRD2 BRD3 BRD4 and BRDT over BD1 in the same BRDs (IC50s >10000 nM for all)
Meso Scale Discovery U-PLEX DEVELOPMENT PACK 25
-PLEX Development Pack, 10-Assay SECTOR Plate (25 Pl)
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay, 10-plate Kit. (96-0046E1CP7L)
The InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRDT(1,2) via detection of BRDT(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRDT(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD3(1,2) Bromodomain Assay, 10-plate Kit. (96-0038E1CP7L)
The InCELL Hunter eXpress HEK 293 BRD3(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD3(1,2) via detection of BRD3(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRD3(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.